MATERIALS AUTHORIZED FOR TREATMENT OF WINES
Proprietors of bonded wine cellars
and others concerned:
Purpose. This circular is to advise you that the Food and Drug
Administration, Department of Health, Education, and Welfare, under the Food
Additives Amendment of 1958, has approved certain pectolytic enzymes, dervied
from Aspergillus niger, produced in accordance with good manufacturing pro-
cedure, for use in the production of wine on and after March 5, 1960; and has
advised that polyvinylpyrrolidone may be used in wine production until further
Background. Industry Circular No. 59-52, dated July 28, 1959, and Industry
Circular No. 59-52, Supplement No. 1, dated August 25, 1959, advised you that
polyvinylpyrrolidone (known as PVP) and certain enzymes (pectinol and pectizyme
[pectinase]) were not subject to the prohibited use section of the Food Additives
Amendment of 1958, until March 5, 1960, and, therefore, their use could be con-
tinued until March 5, 1960. You were also advised that studies on their safety
were in progress.
Materials Authorized. The following materials are considered safe and may
be used, as specified herein, on and after March 5, 1960:
Material Use Limitation
Pectinol 59L To clarify wine. 0.3 pound per 1000 gallons
Klerzyme 200 To clarify wine. One half pound per 1000
gallons of wine.
Pectinol 100D To clarify wine. 1 pound per 1000 gallons
Pectinol 10M of wine.
Pectinase To clarify wine. 10 pounds per 1000 gallons
Concentrate of wine.
Material Use Limitation
Pectinase D Regular
Polyvinylpyrrolidone To clarify and The wine after treatment must
stabilize wine. contain less than one-hun-
dredth of one percent (0.01%)
of polyvinylpyrrolidone.§ 240.524
A proprietor desiring to use any pectolytic enzyme not specified in this
circular must, prior to such use, file a notice and obtain approval therefor in
accordance with regulations in 26 CFR 240.530. The notice shall state the maxi-
mum quantity of the enzyme to be used per 1,000 gallons of wine; and that the
manufacturer of the enzyme guarantees it to be safe and that it was derived from
Aspergillus niger and manufactured in accordance with good commercial pro-
cedure. A four (4) ounce sample of the enzyme must be submitted with the notice.
Inquiries. Inquiries regarding this circular should refer to its number and
should be addressed to the office of your assistance regional commissioner
(alcohol and tobacco tax).
Dwight E. Avis
Director, Alcohol and Tobacco Tax Division
IRS - D. C. - 58213